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BA14-2 could detect a lower concentration of tobramycin than could BA14-1. The data fit well to simple hyperbolic binding curves with a single saturable binding site (Fig. 4). Based on these curves, the apparent Kd for BA14-2 was about 16 μM (R2 = 0.92) while that JNJ-26481585 for BA14-1 was about 500 μM (R2 = 0.87). BA14-2 could reproducibly detect 30 μM tobramycin (p families selected to bind to ATP, members of only one family could detect ATP through changes in fluorescence intensity.
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RNA isolation and characterization Total RNA was
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