Acknowledgments
This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (HK) and from the New Energy and Industrial Technology Development Organization (NEDO) (SW).
Appendix A. Supplementary data
Supplementary Figure 5.
(A) Effects of si-hSNF5 on expression of other SWI/SNF components (hSu(z)12, hbrm, BRG-1 and p250/p250R) were tested. (B) hSNF5 protein level in nuclear extracts from HeLa Cytochrome P450 CYP1B1 (190-19 cool treated with si-hSNF5 multiple times was analyzed. (C) The number of cells treated with different siRNAs as indicated are shown. si-X and si-Y are other control siRNAs. or controls proteins.
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Supplementary Figure 6.
(A) Phosphorylation of chk1-Ser317 and chk2-Thr68 in si-hSNF5-treated HeLa were analyzed. (B) p53 level in nuclear extracts was analyzed as in Fig. 3B. (C) Methylation of ARF and INK4a promoters were tested by treating with aza-C [12]. (D) Cell enlargement was constitutively (TM87-16) or not (HuH7) observed.
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RNA isolation and characterization Total RNA was
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