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Ectopic expression of MRF4 does not induce myogenic differentiation, but does accelerate cell proliferation in myogenic differentiation medium. (A) Representative immunofluorescence images (200× magnification) showing Desmin-positive and DAPI staining (for nuclei) of the BEF-MyoD-Puro and BEF-MRF4-Puro, as well as BEF-Puro control Y-27632 grown in myogenic differentiation medium (DM, DMEM + 2% horse serum + 10 μg/ml insulin) culture conditions for 6 days. (B) Relative growth rates of the BEF-Puro and the BEF-MRF4 cells grown in the DM culture conditions. ??p cell cycle of the BEF-Puro and the BEF-MRF4 cells grown in DM culture conditions for 12 h. ?p