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Jasmonate preparation. (±)-MeJA was purchased from Sigma–Aldrich (St. Louis, MO). Standard conjugate compounds, such as (?)-JA-Ile, (?)-JA-Leu, were prepared from (±)-JA and amino acids according to a previously established method [8]. (±)-Dehydrojasmonic calpain inhibitor 1 (used an internal standard) was prepared according to a previously established method for MeJA synthesis [9]. (±)-D2MeJA, namely methyl [9-2H,10-2H]-jasmonate (double-deuterated at two olefinic positions) was prepared from (±)-methyl dehydrojasmonate with a closed community Lindlar catalyst according to the same method [9] with D2 gas which was generated from CH3OD (Sigma–Aldrich, St. Louis, MO) and Na metal. Atomic% D of the d2JA was determined to be 99.5% by comparison of peak areas of ions at m/z 211 (d2JA) and 209 (JA) by MS analysis. D2JA was converted into conjugates with Ile or Leu [8].
Results and discussion
Achyranthes emits characteristic VOCs upon d2MeJA exposure
Fig. 1.
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