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Effect of β-sitosterol on AMPK signaling
In L6 cells, β-sitosterol (20 μM) treatment increased phosphorylation of the Thr-172 of the AMPK α subunit and the Ser-79 of ACC in a time-dependent manner without changing total protein levels of AMPKα, ACC of β-actin; the phosphorylation level was markedly increased after 2 h, and this increase was maintained for up to 18 h after treatment (Fig. 1C). Treatment with β-sitosterol at concentrations of up to 20 μM increased phosphorylation of AMPKα and ACC, whereas no stimulatory effect on AMPKα and ACC phosphorylation was detected in L6 Hesperadin exposed to 10 μM β-sitosterol for 2 h (Fig. 1D).
Effect of β-sitosterol on glucose uptake and GLUT4 translocation
Fig. 2.
Effect of β-sitosterol on glucose uptake and lipid content in L6 myotube cells. (A) β-Sitosterol (20 μM) treatment for 2 h increased 2-DG uptake in a concentration-dependent manner. (B) Increased GLUT4 translocation to the plasma membrane in L6 myotube cells after 20 μM β-sitosterol treatment for 2 h. (C) β-Sitosterol (20 μM) decreased triglyceride content in L6 myotube cells. (D) β-Sitosterol (20 μM) decreased total cholesterol content in L6 myotube cells. Data compiled from four independent experiments and values are expressed as mean ± SE. ???p