Statistical analysis. Data are presented as mean and standard error of the mean of at least two independent replicates. One-way Analysis of Variance (ANOVA) followed by Tukey’s multiple comparison tests were used to assess statistical significance (P MDYKDHDGDYKDHDIDYKDDDDK transiently transfected with ERα and ERα mutants.
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Ligand-dependent recruitment of ERα to CYP1A1 and CYP1B1
Fig. 2.
TCDD-induced the recruitment of ERα-WT, ERα AF mutants and AHR to CYP1A1 and CYP1B1 regulatory regions. HuH-7 cells were transfected, treated and ChIP assays were performed as described in Materials and methods to detect recruitment of ERα-WT and ERα mutants or anti-AHR (H-211) to (A and B) CYP1A1 and (C and D) CYP1B1 regulatory regions. Percent recruitment levels greater than within similarly transfected cells that was significantly (P
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GTP-Binding Protein Fragment Road verges are considered as refuge
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