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Delayed mineralization in the CCN2-MT LY2090314 and effect of exogenous rCCN2 on the mineralization in both types of osteoblasts
To evaluate bone mineralization in each mouse, von Kossa staining was performed on the tibial and coronal sections (Fig. 4A–D). CCN2-MT cortical bones of tibia showed remarkably delayed mineralization (Fig. 4B). Although mineralized trabecular bones were observed in the WT tibia (Fig. 4A), no structure stained in brown was recognizable in the bone marrow area of the CCN2-MT tibia (Fig. 4B). The degree of mineralization in the frontal bone of the CCN2-MT mouse was also reduced (Fig 4C and D). An examination of skeletal preparations (Fig. 4E–J) demonstrated delayed ossification in the intramembranous skeletal elements of the CC2-MT mice. Both the frontal and parietal bones were properly mineralized in the WT mouse at this time point (Fig. 4G and H). However, interparietal bone mineralization was not clearly observed in the CCN2-MT embryo (Fig. 4E–J).