Fig. 3.
Expression of 14-3-3 ABT-263 in human prostate cancer cell lines. Expression of the seven 14-3-3 genes was detected with gene-specific primer pairs in RT-PCR analysis. Commercial total RNA of a normal human prostate (Prostate) was used as control. Representative results from two experiments are shown.
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We then used mammalian two-hybrid assays to assess the interaction between PrLZ and 14-3-3 proteins. Coding sequences for the five PrLZ isoforms were cloned individually to the pACT and pBIND vectors as fusion proteins. Coding sequences for the seven 14-3-3 proteins were obtained from C4-2 cells by RT-PCR cloning, and transferred to the pACT and pBIND vectors after DNA sequencing. In mammalian two-hybrid assays in C4-2 cells, each PrLZ isoform in pACT was examined for its interaction with the seven 14-3-3 proteins in pBIND. In a complimentary study, each PrLZ isoform in pBIND was examined for interaction with the seven 14-3-3 proteins in pACT.
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Distribution patterns Although both species are considered
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