Figure optionsDownload full-size imageDownload as PowerPoint slide
Fig. 2.
(A) Genomic organization of the murine Sirt4 gene. Repetitive sequences (SINEs and LINEs) are indicated. The sirtuin catalytic domain overlaps the SIRT1 protein region that is encoded by exons 3 and 4. (B) Exon/Intron splice junctions of the murine Sirt4 gene: Huperzine A sequences are given in uppercase and intron sequences in lowercase letters. Exon/Intron sizes are indicated.
Figure optionsDownload full-size imageDownload as PowerPoint slide
Phylogenetic analyses
Phylogenetic trees were constructed from the known SIRT4 protein sequences, which were obtained from a protein sequence similarity search with the murine SIRT4 protein sequence using the BLAST 2.0 program at NCBI database (Non-redundant GenBank CDS: translations + PDB + SwissProt + SPupdate + PIR) and compared to the yeast SIR2 protein (GenPept P06700). Progressive multiple sequence alignments were performed with the CLUSTAL W Multiple Alignment Program Version 1.7 [19]. Trees were calculated and drawn with the PATH (phylogenetic analysis task in HUSAR) software, which estimates and realizes phylogenies by executing the three main phylogenetic methods: distance, parsimony and maximum likelihood and which is hosted by the HUSAR (Heidelberg Unix Sequence Analysis Resources) server from the Biocomputing Service Group at the German Cancer Research Center (DKFZ, Heidelberg) (Fig. 4).
View User's Journal
Distribution patterns Although both species are considered
 |
