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Fig. 1.
Relative binding rate of ssDNA and LPS. (A) CPM of residual radioactive Iniparib on nitrocellulose membrane after screening (X ± s, n = 3), #p 0.05: BSA group vs. initial library, demonstrating no ssDNA enrichment. (B) Electrophoregram of screened ssDNA. With increasing screening rounds, the electrophoresis band gradually became a unitary band, particularly after 9–12 rounds of screening, demonstrating that with increasing screening rounds, aptamers with a high affinity for LPS were significantly enriched.
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Cloning, sequencing, and structural analysis of oligonucleotide aptamers
Fifty positive clones were selected randomly and sequenced to obtain 46 sequencing results. Analysis demonstrated that the primary structures could be classified into: (1) identical sequences, (2) relatively conserved sequences; and (3) non-conserved sequences (Supplemental Table). There were 21 sequences identical to aptamer 19, and the sequences of aptamers 18 and 19 were highly homologous, with only one base difference. Secondary structure analysis of some sequences of each type demonstrated that secondary structure differed significantly between these sequence types, and was usually a hairpin or stem–loop structure (Supplemental Figure).