Antibodies and reagents. Anti-FLAG M2-agarose, FLAG peptide, and rabbit and mouse anti-FLAG
SU5416 were from Sigma (St. Louis, MO). Monoclonal CaMKIV and PP2Ac antibodies were from BD Biosciences Pharmingen (San Diego, CA). CaMKIV phospho-T200 and CaMKIV polyclonal antibodies were from Bethyl Laboratories, Inc. (Montgomery, TX). Generation and characterization of affinity-purified Bα/Bδ antibodies were reported previously [12] and [16]. Secondary antibodies for fluorescence detection were from Rockland (Gilbertsville, PA) or Molecular Probes (Eugene, OR). Normal rabbit IgG was from The Jackson Laboratory (Bar Harbor, ME), and protein A-Sepharose was from Zymed Laboratories Inc. (San Francisco, CA). Lipofectamine 2000 and TransIT-Express transfection reagents were from Invitrogen (Carlsbad, CA) and Mirus (Madison, WI), respectively. Okadaic
acid and ionomycin were from Alexis Biochemicals (San Diego, CA) and Sigma (St. Louis, MO), respectively. The CaMKK inhibitor, STO-609, was from Tocris Bioscience (Ellisville, MO). The Odyssey blocking buffer was from Li-COR (Lincoln, NE).
