STAT3 protects cardiac myocytes from I/R injury. (A) Cardiac myocytes were transfected with 0.5 μg GFP and 0.25 μg STAT3-pcDNA3 or pcDNA3 empty vector for 48 h and subjected to I/R injury for the indicated times. Cell death was measured using TUNEL, a minimum of 200 GFP positive Sitagliptin phosphate were counted per experiment. Experiments were repeated in triplicate, and statistical analysis was carried out using a 2-way ANOVA with a Bonferroni post test, ?p using annexin V and 7AAD staining, ?p autonomic system two experiments.