Figure optionsDownload full-size imageDownload as PowerPoint slide
Peak 3 from the gel Tubastatin A chromatography (Superdex peptide 10/300GL column) was applied to a HPLC reverse-phase C-18 column, fractions were obtained and collected, and the peak fraction was checked for antimicrobial activity (Fig. 2). This peak showed an antimicrobial activity of about 3.2 ± 0.02 MIC μg/ml (Table 2) and this fraction was collected and pooled.
Fig. 2.
High performance liquid chromatography of fractions obtained after gel filtration chromatography. Fraction 3 from Superdex Peptide 10/300 GL was subjected to separation on a HPLC-Reverse C18 column using a linear gradient of acetonitrile (10–95%). The eluate was monitored at 225 nm. All fractions were concentrated through vacufuge and checked for antimicrobial activity.
Figure optionsDownload full-size imageDownload as PowerPoint slide
Table 2.
Antimicrobial activity of ISAMP for Gram-positive and Gram-negative bacteria.
MicroorganismsAntimicrobial activity (MIC μg/ml)
View User's Journal
Fluoxetine is primarily excreted as a parental
 |
