The results presented above demonstrate that the activity of an individual cell-based reconstructed network is changed by tetanic stimulation. The next question, we asked was how long do the tetanus-induced changes persist. To answer this question, we measured the evoked responses 3, 6, and 24 h after tetanus. Fig. 2E and F shows spikes evoked by five test stimuli 3 and 6 h after tetanus. Responses were evoked by each test stimulus, with average latencies of 1.84 ms at 3 h and 1.86 ms at 6 h. The average
MP470 inhibitor of the burst signals evoked 3 and 5 h after tetanus were, respectively, 12.95 and 12.71 ms (Table 1). These values are almost the same as those measured 0.5 h after tetanus and therefore indicate that tetanus-induced changes in activity persist for at least 6 h. At 24 h after tetanus, however, the average latency had increased to just over 5 ms, and the average
period of the burst signals was as long as it was before tetanus (Fig. 2G and Table 1). These results, in the timing of first spike and period of burst signals, suggest hysteretic change from the persistence of tetanus-induced activity.
