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Results
hESC differentiation on various matrices
Undifferentiated hESCs grow in compact colonies, with well-defined round-shaped boundaries as shown in Fig. 1a. Three days after they were re-seeded on the substrates without the MEFS feeder layer or the conditioned media, the TG101209 on gelatin showed random cell morphologies (Fig. 1b), deviating from the small spherical shape of undifferentiated hESCs. However, the cells barely expressed the markers of sox17 (endoderm), brachury (mesoderm) and nestin (ectoderm) for early stage differentiation. With collagen or collagen/CNT as the matrices, more than 90% of the cells elongated and well spread with long filaments (Fig. 1e and h). The cells and their filamental extensions preferentially aligned along one direction due to the dip-coating preparation of collagen or collagen/CNT cell culture matrices, which will be analyzed later. The same alignment was also obeyed by most nuclei, evident in Fig. 1g and j.
Fig. 1.
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