Fig. 1.
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Administration of 17-DMAG and/or PEG-IFN. Starting on day 0, HCV-infected chimeric mice with humanized liver received intravenous injections of 4 mg/kg of 17-DMAG and/or subcutaneous injections of 30 μg/kg PEG-IFNα-2a (Chugai, Tokyo, Japan) according to SCH66336 schedule shown in Table 1.
Table 1.
Schedule of blood sampling and drug administration for chimeric mice infected with HCV
Day?101234567891011121314
Collection of bloodBBB
17-DMAGDDDDDDDDDDDDDD
PEG-IFNIIII
17-DMAG + PEG-IFND/IDDD/IDDDD/IDDD/IDDD
B indicates sampling of blood; D, intravenous injection of 17-DMAG (4 mg/kg); I, subcutaneous injection of PEG-IFN (30 μg/kg).
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Quantification of HCV RNA by real-time PCR. Total RNA was purified from 1 μl of serum from chimeric mice with humanized liver by the acid guanidinium–phenol–chloroform method, and HCV RNA was quantified by real-time PCR as described by Takeuchi et al. [17].
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