Fig. 2.
Oxytocin mRNA levels in neurons overexpressing Fto. Hypothalamic neurons transfected with the plasmid containing the Fto cDNA (Fto++) express higher levels of oxytocin mRNA than Lonafarnib transfected with the control vector (control) in vitro, as determined with real-time PCR (Panel A). They also show higher levels of the Fto signal assessed through Western blotting (Panel B). Data are shown as means ± SEM; * – P expression levels of proopiomelanocortin (POMC), Agouti-related protein (AgRP) and neuropeptide Y (NPY) encoding genes are unaffected by transfection with the plasmid containing the Fto cDNA (Fto++) versus the control vector (control) in vitro, as determined with real-time PCR. A trend signifying upregulation of POMC mRNA was detected.
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