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Both NM23-H2 and Bcl2L10 co-localize in the cytoplasm
NM23-H2 down-regulates Bcl2L10 expression
Transfection of increasing concentrations of Diva expression plasmid augmented the expression of Diva protein as determined by immunoblotting with anti-Diva CEP18770 (Supplementary Fig. 1A). However, when cells were co-transfected with NM23-H2 along with Diva, cells expressed significantly lower levels of Diva compared to cells that were not overexpressing NM23-H2 (Supplementary Fig. 1A; upper panel, lanes 2–6 vs. 8–12). Furthermore, we confirmed that the down-regulation of Diva protein is mediated by NM23-H2 through the use of NM23-H2 knock down cells. Silencing of NM23-H2 was evident as shown by Western blot analysis in Supplementary Fig. 1A (middle panel, lanes 13–1 cool . When Diva was overexpressed in the NM23-H2 knock-downed cells, the expression levels of Diva were not decreased but unaltered (Supplementary Fig. 1A; upper panel, lanes 14–18 vs. 2–6 and 8–12), indicating that NM23-H2 is responsible for down-regulating Diva protein levels in the cells.
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