Establishment of the uPA-NOG mouse as a model of spontaneous hepatic injury. (A) The uPA
Geneticin unit contains the mouse albumin enhancer/promoter (Alb En/Pro), the chimeric intron, mouse uPA cDNA, and the 3′-UTR of the human growth hormone gene with polyadenylation (pA) signal. Arrowheads depict the positions and directions of the RT-PCR primers. (B) RT-PCR analyses of uPA transgene expression. Wild, non-transgenic NOG; Tg/+, hemizygous; and Tg/Tg, homozygous uPA-NOG mice. Gapdh was used as an internal control. (C) ALT activities in uPA-NOG mice. All the values for the homozygous uPA-NOG (Tg/Tg) are significantly higher than those for the hemizygote (Tg/+) (P
hemizygous and homozygous uPA-NOG mice represent the mean ± SD of four to seven samples. (D) Gross appearance and histopathology of a homozygous uPA-NOG mouse liver. Scale bar, 100 μm; P, portal tract; C, central vein.
