To examine the effects of α-catenin overexpression on osteoblastic differentiation,
Lisinopril dihydrate were treated with Wnt-3A CM for 72 h. As shown in Fig. 1A, C3H10T1/2 cells overexpressing α-catenin exhibited markedly increased ALP activities compared to empty virus (GFP)-transduced cells. Since Wnt-3A CM treatment is a very strong and artificial stimulator of osteoclastogenesis and it requires β-catenin for its activity, we next examined the effects of α-catenin during culture in OM, a more physiological condition for osteogenesis. As shown in Fig. 1B, the enhanced ALP activity was also observed when we treated the
cells with OM for 20 days. ALP staining results on day 20 in OM and on day 3 in Wnt-3A CM are also shown. Real time qPCR analysis also showed that overexpression of α-catenin resulted in increased expression of Runx2 (Fig. 1C) and osteocalcin (Fig. 1D) after culture in Wnt-3A CM compared with that from control cells (GFP) although the difference was marginally significant (p = 0.05 for both Runx2 and osteocalcin on day 3). Taken together, these findings strongly suggest that α-catenin promotes osteoblastic differentiation of C3H10T1/2 cells.
