Since PL and PD were difficult to measure directly by experiment, factors that
AZ 960 might affect PL and PD were analyzed. Larval motility might be an important underlying factor. Larval motility in light and dark conditions was evaluated using a 2-min midline accessing test. The 2-min tests in which the larvae started from a starting line far from (RMF) or near to (RMN) the LD boundary were performed for under- and overestimations of larval motility respectively. As shown in Fig. 2C, the RMF and RMN in the light/dark conditions before the 10-min phototaxis test were not significantly different. After the 10-min phototaxis test, the midline accessing rate (RM) with larvae averagely starting from the center of one testing plate half was used to estimate motility. Here, RM, but not RMF and RMN, was tested to minimize the arousal effect of brush disturbance on larval motility. After the 10-min phototaxis test, a
ionic bond more drastic difference between motility in the dark and light conditions was observed ( Fig. 2C).
