Statistical analysis. Embryo developmental data were analyzed by t-test using SPSS 13.0 software. The level of significance was set at P
JNJ-7706621 stage. Untreated polyspermic zygotes were used as controls. Our results indicated that there was no significant difference in preimplantation development between the nocodazole treated and untreated polyspermic zygotes (Table 1). In 92 zygotes arrested in mitosis, 80.4% survived after enucleation. Of these surviving zygotes, 55.4% survived after nuclear transfer and 46.3% cleaved to at least the 2-cell stage (Fig. 2A). The developmental potential was limited to the 8-cell stage (Fig. 2B). However, when
interphase polyspermic zygotes were used as recipients for nuclear transfer, development of the embryos arrested at the first cleavage (Table 2).
