LPS used in this Safinamide Mesylate work (smooth LPS, sLPS) has linked to lipid A a long polysaccharide chain (O-antigen) which also provide an effective steric barrier for inhibiting detergent interaction.
FITC-LPS distribution in GUVs showing macrodomain segregation
In order to observe the distribution of LPS in the membrane, GUVs of the mixture DOPC biggrin PPC:FITC-LPS (1:1:1 molar ratio) were observed at two temperatures. Fig. 2 shows the intensity image taken from the top surface of one of these GUVs. The surface appears homogeneously fluorescent. Since the only fluorescent molecule in the chamber is the FITC-LPS (control experiments with unlabeled LPS show no signal) this result indicates that LPS is effectively incorporated into lipid bilayer during the GUVs formation and moreover it shows that, at temperatures at which the two lipids are over their Tm, LPS is homogeneously distributed in the bilayer. At 25 °C, however a preferential location of FITC-LPS molecules in some specific areas of the GUV can be observed ( Fig. 3A).
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