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Transgene-expressing periosteal KU-57788 express master transcription factors for chondrocyte and osteoblast differentiation and receptors for FGF, Ihh, and PTH
Chondrogenic and osteogenic differentiation of transgene-expressing periosteal cells
Fig. 3.
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We also examined the osteogenic potential of GFP-positive and -negative cells by inducing osteogenic differentiation with β-glycerophosphate and ascorbic acid. After 4 weeks of induction, GFP-positive cells showed more intense staining for alkaline phosphatase activity compared with GFP-negative cells (Fig. 3F). GFP-positive cells also showed more intense staining with alizarin red, indicating increased matrix mineralization (Fig. 3G). Semi-quantitative PCR analyses of GFP-positive cells indicated that osteogenic induction caused increased expression of osteoblast markers Runx2, Osterix (Osx), Bone sialoprotein (Bsp), and Alkaline phosphatase (Alp) ( Fig. 3H). These observations indicate that GFP-positive cells can differentiate into osteoblastic cells in vitro.