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Despite the significant differences in the mitochondrial energy state, the total cellular ATP content is the same in the two cell lines (data not shown), implying that glycolytic phosphorylation has a higher share in ATP production in SC-5 than in IMO cells. Indeed, the much higher rate of lactate formation by SC-5 PF-562271 incubated with 5 mM glucose in comparison to that found for control myoblasts (2866 ± 117 vs. 861 ± 428 nmole/mg protein/30 min, p anaerobic ATP formation.
Fig. 4.
Mitochondrial complexes III and V content in SC-5 and IMO cells. (A) Western blot of mitochondrial complexes III and V and β-actin loading control (typical data from one out of three experiments). (B) Densitometric analysis of blots data for these mitochondrial complexes (n = 3). ?p
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Fluoxetine is primarily excreted as a parental
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