Glucose transport. Glucose transport was studied on hMADS adipocytes starved overnight in DMEM containing 0.2% BSA. Cells were then incubated in Krebs–Ringer phosphate buffer containing 0.2% BSA [3] for 4 h at 37 °C followed by treatment for 45 min with insulin (100 nM) and 2-Deoxy-d-[2,63H] glucose (Amersham, Piscataway, NJ, USA) was added during the last 10 min (100 μM of 2-Deoxy-d-glucose containing 0.5 μCi/ml of the tritiated form). Glucose uptake was stopped by washing the
apexbio with ice-cold PBS followed by cell lysis in PBS containing 1% triton. Statistical analysis. Results are shown as mean ± standard error of the mean (SEM) with the number of experiments indicated. Continuous variables and their change from control conditions were analysed with Student t-test using Micrococal Origin 6.0 (Micrococal Software, Northampton, MA). Probabilities values
dystrophin accumulated into lipid droplets [14]. LPV or RTV treatment of hMADS adipocytes, neither induced any morphologic change as compared to control nor modified the Glycerol 3-phosphate dehydrogenase (GPDH) activity indicating that metabolic changes were unlikely to result from an alteration of the differentiation state of hMADS adipocytes (see Supplemental data).
