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Introduction
However, most information about transcription factor VER155008 involved in the mechanical response is derived from studies with endothelial cells [3]. In some genes, such as PDGF-α and -β, a shear stress responsive element (SSRE) was identified, which can positively or negatively regulate gene expression in response to fluid shear stress. So far only a few transcription factor binding sites, which are responsible for the activation of gene transcription in osteoblastic cells by mechanical loading have been identified.
In the present study we investigated the influence of mechanical strain on the expression of HB-GAM and its regulation by mechanical strain in the human bone marrow mesenchymal stem cell line hMSC-TERT4. This cell line has been created by stable transduction of the human telomerase reverse transcriptase gene. hMSC-TERT4 cells are capable of adipocytic, chondrogenic and osteoblastic differentiation under the appropriate culture conditions [12]. We were able to show that HB-GAM expression is upregulated by mechanical strain in hMSC-TERT 4 cells. In this manuscript, we describe the transcriptional regulatory region in the 5′ flanking promoter of the HB-GAM gene, which mainly contributes to the mechanical-induced HB-GAM expression.