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Fig. 4.
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Acknowledgments
We thank Weibo Xie and Lei Wang for microarray data analysis; Professors Caiguo Xu and Xianghua Li for field management and technical aids and Professor Qifa Zhang for support. This work was supported by grants from win 55 212-2 Chinese Ministry of Science and Technology and the National Natural Science Foundation.
Appendix A. Supplementary data
Supplementary Fig. 1.
Expression analysis of HDAC over-expression and RNAi transgenic plants. (A) Expression analysis of HDA706, HDA711, HDT701 and HDT702 over-expression plants by RNA gel blot hybridization. (B). Expression analysis of HDT701 RNAi plants by quantitative RT-PCR.
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Keywords
Drought stress; Expressed sequence tag; MicroRNA; MicroRNA targets; Populus euphratica
Introduction
MicroRNAs (miRNAs) are endogenous non-coding small RNAs, typically about 21 nucleotides in length, that repress gene translation or degrade target mRNAs and therefore have negative regulatory functions at the post-transcriptional level [10] and [11]. A great deal of effort has gone into the identification of miRNAs in two model plants, Arabidopsis and rice. In the recently published miRBase 13.0, 112 and 143 miRNA families were described in Arabidopsis thaliana and Oryza sativa, respectively, whereas only 42 such families have been found in P. trichocarpa. Furthermore, most poplar miRNAs in the miRBase have been identified by comparing obtained sequences with genus-conserved miRNAs in A. thaliana and other genera, even though the majority of poplar miRNAs are genus- or species-specific [12]. Consequently, identifying novel and functional miRNA families in Populus have become of interest.