Fig. 2.
Kinetic analysis of wild-type (WT) GLuc luminescence specific activity compared with variants. For each plot, the WT GLuc profile is represented by a solid black line with diamonds. (A) Kinetic profiles of GLuc mutants with replacement of YM155 residues with either AHA or HPG. (B) Kinetic profiles of GLuc variants with single mutations of methionine residues to leucine. (C) Kinetic profile of GLuc variants with multiple methionine to leucine mutations.
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Table 1.
CFPS yields, specific and total activities, and half-lives of emission for wild-type (WT) GLuc as well as for each mutant tested. All values were determined in triplicate. Average values and one standard deviation are amensalism shown.
GLuc total yield (μg/mL)GLuc soluble yield (μg/mL)Specific activity (×1023 photons/s/mol)Total activity (×1016 photons/s/mL)Half-life (min)
WT GLuc451 ± 105416 ± 1779.0 ± 3.12.0 ± 0.31.3 ± 0. 3
AHA mutant460 ± 97220 ± 404.4 ± 1.70.5 ± 0.11.8 ± 0.5
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Such low level of genetic differentiation
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