Welcome to Gaia! :: View User's Journal | Gaia Journals

Costimulation; B7 family; Contact hypersensitivity; Mouse; T cells
Introduction
Increasing number of studies using human tumor samples have revealed that B7-H4 is overexpressed in various tumors, including breast, ovarian, renal, prostate, and non-small-cell lung cancers, and that B7-H4 Sulfadimethoxine assessed using RT-PCR and immunohistochemistry is associated with disease progression [6], [7], [8], [9] and [10]. In addition, B7-H4 is expressed in tumor-associated suppressive macrophages [11] and serum soluble B7-H4 is also elevated in patients with renal cell carcinoma and ovarian cancer [12] and [13].
In this study, we generated a new mAb against mouse B7-H4, reassessed B7-H4 expression and function in immune cells, and examined the involvement of B7-H4 in T cell-mediated immune responses.
Materials and methods
Mice. Female 6-week-old BALB/c mice were purchased from Japan SLC (Hamamatsu, Shizuoka, Japan) and maintained under specific pathogen-free conditions. All procedures were reviewed and approved by the Animal Care and Use Committee of Tokyo Medical and Dental University.