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Fig. 4.
Concentration dependence conversion of SAA2.2 octamer to hexamer. SAA2.2 was purified and dissolved in buffer A (20 mM Tris, 6 M urea, 400 mM NaCl, WP1066 cool and aliquoted at three different concentrations (A: 0.25 mg/mL, B: 0.8 mg/mL and C: 1.8 mg/mL) and allowed to refold at 4 °C for 14 h in 500–1000× refolding buffer (20 mM Tris, pH cool . SAA was aliquoted in sterile tubes and stored under refrigerated conditions. At regular intervals (as specified in the figure) samples were analyzed on a Superdex 200? analytical gel filtration column.
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Acknowledgment
This work was supported by a Grant from the NIH (R01 AG02815 cool to W.C. The AUC experiments were performed in the Analytical Biochemistry core facility of the Rensselaer Center for Biotechnology and Interdisciplinary Studies.
Keywords
T-817MA; Neuroprotective; Tau; Transgenic; Tauopathy; Alzheimer’s disease
1. Introduction
2. Materials and methods
2.1. Animals and treatment protocol