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Fig. 5.
Detection of exogenous ecdysteroid activity using eggs expressing the reporter gene. The eggs were injected with the reporter plasmid DNA and exposed to 20E. After 12 h, GFP signals were measured by using three eggs that PR619 were independently injected. Three images were obtained from each egg, and the average intensity and standard error (S.E.) were calculated. Bars indicate S.E.s. Asterisks above the bars indicate significant differences (*P embryo of daphnids and that it could be used to monitor active ecdysone. This reporter gene method can be used to detect the presence of exogenous ecdysone, anti-ecdysone activities, or endogenous ecdysone activity. Further strategies to develop transgenic lines of Daphnia expressing reporter genes would be beneficial for the evaluation of ecdysone activities both within animals and in the environment.
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Fluoxetine is primarily excreted as a parental
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