Supply Of Therapeutic Agents By Intracerebroventricular (ICV) And Intravenous (IV) Injection In Mice
Despite the protecting role that blood mind barrier performs in shielding the brain, it limits the access to the central nervous system (CNS) which most often results in failure of potential therapeutics designed for neurodegenerative disorders 1,2. Place the needle diagonally within the microcentrifuge tube containing the injection answer. Load the injection resolution into the needle by fastidiously pulling the plunger of the syringe. ICV injection: The mind may be harvested at completely different time points submit-injection and visualized to demonstrate successful injection technique. If the dye is not added to the injection solution, it will be tough to check for the accuracy of the injection. In this case, the depth of the needle was not sufficient and the injection answer has probably distributed under the skin. IV injection: Accurate and environment friendly temporal/facial vein injection will be visualized instantly following the injection.
Moreover, altered skin coloration of the mouse shall be observed following injection. For easy visualization of injection and through a follow interval, a 1:50 dilution is really useful. An inaccurate injection will result within the accumulation of inexperienced dye under the skin on the site of the injection. A) Schematic of injection websites utilized in I.C.V. injections into the ventricles. B) Mind from I.C.V. injected Pup with trypan blue in PBS on left ventricle, photograph taken quarter-hour publish injection.
C) Brain from I.C.V. injected Pup with trypan blue in PBS on left ventricle, photo taken 60 minutes put up injection. B) Pup injected I.V. with inexperienced dye in PBS compared to un-injected littermate, Picture taken immediately submit injection. On this video, we demonstrate the step-by step procedures involving two types Enhanced ICV of injection strategies that can be utilized to target the CNS: 1) direct injection into the CNS utilizing intracerebroventricular (ICV) injection; or 2) IV injection concentrating on the temporal/facial vein.
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