Acknowledgments
This work was granted by the “Ministère de la Recherche et de l’Enseignement” and by the “Association de la Recherche contre le Cancer”.
Appendix A. Supplementary data
Supplementary Figure S1. Figure optionsDownload full-size imageDownload as PowerPoint slide
Keywords
Adipocyte; Nuclear factor of activated T cells; NFAT; Lipolysis; Gene expression; Cyclosporin A; CsA; Glycogen synthase kinase 3β; GSK3β; TNFα
The immunosuppressant cyclosporin A (CsA) inhibits the phosphatase activity of Cn through formation of Cabozantinib malate (XL184) stable complex with cyclophilin [1]. CsA prevents NFAT nuclear localization and can be used as a pharmacological probe to uncover novel functions of the Cn/NFAT pathway. Treatment of preadipocytes with CsA effectively blocked adipocyte differentiation, implicating NFAT activity in adipocyte differentiation [5]. Another recent report indicated that stable expression of phosphorylation-resistant Ala-168/170-NFAT3/NFATc4 facilitated adipocyte formation of NIH 3T3 cells under differentiation conditions and was accompanied with increased PPARγ expression [14].
View User's Journal
Statistical analysis Statistical analysis was performed using GraphPad Prism Software
 |
