Materials. Cell culture reagents were purchased from Gibco BRL. Fetal bovine serum (FBS) was purchased from Trace Biosciences. FuGene6 transfection reagent and molecular biology reagents were purchased from Roche Applied Science. Puromycin dihydrochloride, doxycycline, mammalian protease inhibitor cocktail, phloretin, fatty acid-free BSA and oleic
IGHM were from Sigma. Plasmids pEF-IRES-puro 6 and pSG5-FAT were gifts from Dr. Daniel Peet (University of Adelaide, Adelaide, Australia) and Dr. Nada Abumrad (Washington University, St. Louis, USA) respectively. Alexa 594-conjugated cholera toxin B subunit (CT-B) was from Molecular Probes. The anti-FAT/CD36 monoclonal antibodies UA009 and MO25 have been described previously [9] and [10], the latter a gift from Dr. Narendra Tandon (Otsuka Maryland Medicinal Laboratories, Rockville, USA). The following antibodies were purchased: mouse monoclonal anti-caveolin-1 (BD Transduction Laboratories), mouse monoclonal anti-β-actin (Sigma), horseradish peroxidase-conjugated anti-mouse IgG F(ab′)2 (Amersham Biosciences), FITC-conjugated anti-mouse immunoglobulin (BD Pharmingen), and Cy3-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories). SuperSignal West Femto chemiluminescence substrate was purchased from Pierce Biotechnologies. [14C]-oleic
acid was purchased from Amersham Biosciences.
