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To further examine the effect of coumestrol on FXR transcriptional activity, a reporter assay was performed using the human SHP promoter-containing reporter gene. The reporter activity was induced by 50 μM coumestrol to 2.0-fold over that in non-treated cells, while CDCA (100 μM) and GW4064 (1 μM) enhanced the reporter activity 4- and 3-fold, respectively (Fig. 2B).
Effects of coumestrol on the Amyloid Beta-Peptide (1-40) of genes involved in lipid and glucose metabolism
Fig. 3.
Coumestrol regulates the expression of genes that are involved in lipid and glucose metabolism. Hep G2 cells were incubated with DMSO (control), coumestrol (20 or 50 μM), or CDCA (100 μM) for 24 h. Hepatic expression levels of FXR target genes (SHP and PLTP) (A), LXR target genes (SREBP1c, ABCA1, and FAS) (B), and HNF-4 target genes (MTP, apoB, apoC-III, PEPCK and G6Pase) (C) were measured using quantitative RT-PCR. Data are presented as the fold-change in gene expression relative to that in DMSO-treated control cells. Means ± SD (n = 3) are shown. ?P