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Acknowledgments
We thank Dr. Pompon for his gift of Yeast WAT11 and WAT21 cells. This research was supported, in part, by grants from the Plant Diversity Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology of the Korean Government (PF06304-01), the Plant Metabolism Research Center at Kyung Hee University, Science Research Center Program from the Korea Science and Engineering Foundation, RO4987655 Korea Research Foundation Grant funded by the Korean Government (MOEHRD, a Basic Research Promotion Fund, KRF-2005-070-C00129) to S.C., and a Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to S.F. (Grant No. 19380069). B.K. was supported by a BK21 Research Fellowship from the Ministry of Education, Science and Technology.
Keywords
Pex5p ubiquitination; Pex10p; Ring E3 ligase; Ubc4p; Pex4p; Peroxisomal matrix protein import
Peroxisomes are diverse single membrane bound organelles that can be found in all eukaryotic cells. Peroxisomal enzymes are synthesised in the cytosol and imported with the aid of a peroxisomal targeting signal (PTS) [1]. The most common signal is the PTS1, located at the extreme C-terminus and consisting of variants of the canonical S-K-L sequence of firefly luciferase [2]. The less common PTS2 is an N-terminal nona-peptide, with the consensus (R/K)(L/V/I)X5(H/Q)(L/A) [3]. The import of PTS containing proteins requires the action of a cycling import receptor: Pex5p for PTS1 proteins [2] and Pex7p for PTS2 proteins [3]. A number of co-receptors for the PTS2 pathway, the Pex20p family of proteins, have also been identified [4].