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Acknowledgments
We thank S.Y. Park, Y.H. Yoon and F. Kawai at Yokohama City University for assistance during data collection at beamline 17A of the Photon Factory, Japan. We thank H.S. Lee, K.H. Kim, and K.J. Kim for assistance during data collection at beamline 6B of Pohang Light Source, Korea. Functional Proteomics Center, 21C Frontier Program, of the Korea Ministry of Science and Technology (M108KM010031-08K1301-03111) and the National Institute of Agricultural Biotechnology (05-4-11-16-3). K.H. Nam DZNeP supported by Brain Korea 21 project.
Keywords
Injectable; Biodegradable; Alveolar bone substitute; Cytotoxicity; L929 cell; Unsaturated polyphosphoester; β-Tricalcium phosphate; Component; Cross-linked
Materials and methods
Reagents. BPO was obtained from Shanghai Chemical Reagents Company (Shanghai, China) and purified by recrystallization by standard method. DMA was purchased from Acros (Pittsburgh, PA). NVP was obtained from Merck Company (Rahway, NJ, USA) and purified by distillation. NaCl was purchased from Aldrich (Milwaukee, WI) and sieved to obtain particles in a 100–200 nm size range. UPPE and β-TCP particles (100 mesh) were synthesized by Huazhong University of Science and Technology using established techniques described previously [11] and [14]. Chemical structures of the products were verified by nuclear magnetic resonance (NMR, Bruker, Billerica, MA). The sterilization of the samples was conducted with ethylene oxide.