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Adiponectin suppressed SREBP1c expression via activating AMPK in hepatocytes
Fig. 4.
Inhibition of AMPK phosphorylation abolishes SREBP1c suppression by adiponectin. (A, upper panel) Gene CAL-101 of LKB1 was performed by adenovirus-mediated Cre expression in LKB1lox/lox mice. The panels show the representative blot and quantification of the phosphoAMPK/AMPK intensity of immunoblot analysis. Each bar represents the mean ± SE (subjects, n = 5; control, n = 4; ?P cells were incubated with adiponectin under adenovirus-mediated overexpression of LacZ or DN-AMPK. The panels show the blot of the phosphoAMPK/AMPK. (B, lower panel) The mRNA expression of SREBP1c was measured in the Fao cells after 8 h incubation with adiponectin. Each bar represents the mean ± SE (n = 3, ?P
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