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Fig. 6.
EEM spectra, fluorescence quantum yields (Φ) and Large T antigen - rhesus polyomavirus 560-568 spectra for selected stations exhibiting major, distinct UV emission bands (λex/λem = 300/340 nm (A); 300/405 nm (C); 320/380 nm (E)). The aCDOM data for station 89 (D) was collected employing the 10 cm instead of the 200 cm pathlength as for stations 68 (B) and 58 (F). (A, B) (Station 68: 1.0° N, 0.0° W); (C, D) (Station 89: 2.0° S, 5.0° E); (E, F) (Station 58: 1.65° S, 0.0° E).
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Except in cases where distinct UV emission bands were observed, the spectral dependence of fluorescence quantum yields showed a very similar trend to that observed for coastal and terrestrial CDOM samples (Fig. 5,C–F–I) (Boyle et al., 2009, Green and Blough, 1994, Hoge et al., 1993, Ma et al., 2010 and Vodacek et al., 1995). Fluorescence quantum yields (Φ) increased from λex = 280 nm to a maximum at λex ~ 370/380 nm, ranging from 1.8 to 2.5% (Fig. 5,C–F–I), and decreased monotonically thereafter. However, when present, quantum yields for the distinct emission bands (Fig. 6) were much higher (5 to 7%). No obvious concomitant increase in absorption was observed at excitation wavelengths corresponding to these more highly fluorescent bands (Fig. 6), implying that they contribute little to the total absorption at these wavelengths.
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Immunohistochemistry and assessment Antibodies used were as follows IL goat
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