(A) Real-time RT-PCR results for BTG4 expression in the gastric cancer cell lines, with or without DAC treatment. Results are shown relative to a value of 1 assigned to mock-treated GES-1 cells after normalized to
ASA404 expression. ?P edges of each box represent the 75th and 25th percentile, respectively; the upper and lower bars indicate the highest and lowest values determined, respectively. P = 0.002, two-sided t test. (C) Relative BTG4 mRNA expression in gastric cancer stratified by BTG4 promoter methylation. BTG4 mRNA levels and promoter methylation were determined by real-time RT-PCR and MSP, respectively. Depending on each tumor’s promoter methylation status, the cases were subdivided into two groups, unmethylated (n = 10), methylated (n = 2
cool . Note significantly lower mean BTG4 mRNA
expression in the tumors with promoter methylation (P = 0.012, two-sided t test). (D) MSP for the BTG4 and p16 genes was performed using unmethylation-specific (U) and methylation-specific (M) primer sets. Twenty microliters of PCR product was run on 2% metaphore agarose gel, stained with ethidium bromide and visualized under UV illumination. The numbers shown are sample identification numbers.
