Route 38 is an east-west route located in west Johto. The western terminus is at Route 39 near MooMoo Farm and the eastern end is located at a guard house on the western edge of Ecruteak City.

Even though Route 38 maintains a pure east-west alignment as a whole, there are no less than four separate paths that Trainers can take between Ecruteak and Route 39.The upper middle path is a branch of the northern path, separating near Dana and merging with the northern path near Route 39. There are no trainers along this short stretch; there is, however, a patch of grass that is home to several species of wild Pokémon.nlike the two upper paths, which allow for two-way traffic, the lower paths are configured to allow only one direction of passage. The lower middle path is designed for travelers heading west to Olivine City and is a viable alternative to the grass-lined northern path. The path heads due west from the guard house and travels down a fence-lined walkway that is home to one trainer. Past this trainer, there is a Berry bush just before a ledge that overlooks a grass-lined pathway. After hopping this ledge, trainers are forced to head north along this passageway, encountering another trainer before merging into the upper middle path.The southern-most path is an "express route" from Olivine to Ecruteak and is designed so that only eastward movements are permitted. After breaking from the northern path near Route 39, the path heads south, passing a signpost before traversing a ledge that borders a T-intersection. At this junction, trainers can either head north into the field of grass along the lower middle path or continue east along the southern edge of the ledge of the lower middle path and, eventually, along the fenced area to Ecruteak.