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Statistical analysis. A two-factor ANOVA with repeated measures and Newman–Keuls post hoc test was used to compare the effects of Angiotensin 1/2 (5-7) (control versus pair compressed specimens at 0.0125–0.5 MPa) and zone of origin (inner and outer zones). In the second set of experiments, two-factor ANOVA with repeated measures and Newman–Keuls post hoc test was used to compare the effects of compression (control versus 0.1 MPa) and the various COX/NOS inhibitors.
Results
Effect of compression on PGE2 synthesis
Dynamic compression significantly increased PGE2 production with increasing compression at most magnitudes of stress for the inner zone (Fig. 1A) and all magnitudes of stress for the outer zone (Fig. 1B). Significantly greater PGE2 production was observed in the inner as compared to the outer zone at 0.2 MPa only (p compression induced expression of COX2 protein ( Fig. 2).
Fig. 1.
PGE2 production from (A) inner and (B) outer zone meniscal explants and NO production from (C) inner and (D) outer zone meniscal explants subjected to 24 h of dynamic compression at magnitudes from 0.0125 to 0.5 MPa and a frequency of 0.5 Hz (mean ± SEM, expressed as percent of control from the same zone, ?p