In sum, we show that the majority of oxytocin neurons in the male and female mouse Lonafarnib coexpress Fto. This transcription co-activator up-regulates oxytocin mRNA levels. The relationship between Fto and oxytocin could thus be an important mechanism through which Fto exerts its effect on energy balance.
The studies were supported by the Swedish Research Council (Medicine), Swedish Brain Research Foundation, Novo Nordisk Foundation, National Institute of Drug Abuse, and National Institute of Diabetes and Ingestive and Kidney Diseases. We thank Kedar Ghimire for technical help with Western blotting.
Appendix A. Supplementary material
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Protein tyrosine phosphatase; Trypanosoma cruzi; Chagas’ disease; Recombinant expression
1. Introduction
Protein tyrosine phosphatases (PTPs) form a large family of proteins in eukaryotic genomes with a wide range of functions [1]. Solution of the structures of several PTPs has established an enzymatic mechanism where a catalytic cysteine from the core catalytic motif (H/V)C(X)5R(S/T) (Supplementary Fig. S1) acts as a nucleophile to form a covalent thiophosphate intermediate with the substrate. This intermediate is additionally stabilized by a catalytic arginine and hydrolyzed with the help of a catalytic aspartate, which functions as a general acid/base [1] and [2].