Acknowledgments
This work was supported by the Korea Research Foundation Grant (KRF-2006-311-E00067) funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) and by VX-222 Grant (R01-2006-000-10451-0) from the Basic Research Program of the Korea Science and Engineering Foundation to J. Bae, and by Seoul R&BD grant to K. Lee.
Appendix A. Supplementary data
Supplementary Figure. Figure optionsDownload full-size imageDownload as PowerPoint slide
Supplementary Figure. Figure optionsDownload full-size imageDownload as PowerPoint slide
Keywords
RNA; Aptamer; Beacon; In vitro selection; Tobramycin; Fluorescence
Fig. 1.
Comparison of two types of “structure-switching signaling aptamer”. Ligand binding to a Devonian beacon aptamer disrupts an intramolecular duplex. Signaling through the duplex-to-complex structure-switching mechanism requires disruption of an intermolecular duplex. In both strategies, structure-switching separates a fluorophore from a quencher resulting in increased fluorescence intensity.