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Conclusion
Acknowledgments
The work was supported by the Inserm-Fiocruz collaborative programme, the Institut National de la Santé et de la Recherche Médicale (U547), the Institut Pasteur de Lille, the Centre National de la Recherche Scientifique and the Microbiology program of the Ministère de l’Education Nationale, de la Recherche et de la Technologie (MENRT). The financial support from FIOCRUZ/PDTIS (GO), PAPES (GO 400315/2006- cool and FAPEMIG (DB CBB-174/02) MCOPPB trihydrochloride also acknowledged. F.L. is supported by an NIH-Fogarty grant (TW007012-01). G.O. is a CNPq fellow.
Appendix A. Supplementary data
Supplementary Fig. 1.
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Keywords
Resveratrol; Cardiomyoblast; Differentiation
Materials and methods
Cell culture. H9c2 cells obtained from the were grown as a stock of cells in culture flasks in Growth Medium (GM) in which DMEM was supplemented with 10% fetal calf serum (FCS) (Gibco) and 1% penicillin/streptomycin (Gibco). Myogenic differentiation was promoted by changing subconfluent (80%) cells to Differentiation-enhancing medium (DM) in which the DMEM contained 1% FCS. Resveratrol was supplied by Sigma (St. Louis, MO) and the stock solution was prepared in ethanol. In GM group, cells receiving ethanol (0.25%) served as a vehicle control.