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Activation of Akt is required for CTGF-mediated increase in cell size
So far, it CEP-18770 has been reported that the activation of MAPK family is a critical event in the onset of cardiac hypertrophy. Therefore, we examined the importance of ERK1/2, p38 MAPK, or JNK in CTGF-mediated increase in cell size by using their specific inhibitors, U0126, SB202190, or SP600125. As shown in Fig. 4A, CTGF-induced increase in cell size was not affected by these inhibitors, suggesting that MAPK family pathways are unlikely to be necessary for cardiomyocyte hypertrophy in CTGF signaling.
Fig. 4.
Dominant negative Akt inhibits CTGF-induced cardiac hypertrophy. (A) Cardiomyocytes were subjected to full length of 1.3 nM CTGF with 5 μM U0126, 5 μM SB202190 or 1 μM SP600125, respectively, and the cell size was quantitatively analyzed as described in Fig. 1. Data are Paleozoic Era shown as the means ± SE, ?P