Inoculation of plants with the blast fungus Magnaporthe grisea. The preparation of fungi, and inoculation of plants with the incompatible race 102 (84–107B) and the compatible race 001 (kyu91–107) of M. grisea were described previously [3]. The inoculated leaves were harvested at the indicated time points for antidepressant amphetamine analysis. Seven days after inoculation, the leaf lesions were classified into four levels of disease severity as follows. Level 1: when M. grisea infection had failed, the inoculation spot became a small white scar, similar to that resulting from a mock-inoculation. Level 2: a small dark-brown lesion had formed at the inoculation spot. Level 3: the dark-brown lesion had spread and surrounded the inoculation spot. Level 4: chlorotic and stunted regions had spread across the leaves.
Results
Characterization of rice OPR3-homologous genes
Table 1.
The OPR homologous genes in rice
New nameLocus IDaKnown namebAccession Nos.Similarity (%)
OsOPR3OsOPR1AtOPR3AtOPR1AtOPR2
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GTP-Binding Protein Fragment Road verges are considered as refuge
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