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Since pH homeostasis is relevant to central nervous system (CNS) function and physiology, we investigated the expression of TDAG8 in rodent brain. Here, we report the cloning and acid-sensing capability of TDAG8 receptor predominantly expressed in rat forebrain regions. Pharmacological studies reveal that Balicatib TDAG8 receptor acts as an acid-sensing receptor coupled to specific cell signaling pathways. Our observations open new areas of investigation studying contributions of brain TDAG8 to cerebral pH homeostasis and perhaps pathological states associated with central pH malfunction.
Materials and methods
Animals. PCR template was generated from adult male Sprague–Dawley rats (Charles River; 250–300 g). Animals were maintained in constant temperature/humidity vivarium with standardized lighting and free access to chow and water. Procedures were approved by the institutional animal care and use committee (IACUC).
Primers corresponding to bp 7–29 and bp 977–1000 of the TDAG8 sequence were used for TDAG8 detection in various brain regions. PCR products were separated by electrophoresis on 1.2% agarose gel and stained with ethidium bromide (primer sequences and other details are available in the online supplement).