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1. Introduction
Although there is substantial evidence for widespread CDOM photobleaching from its distribution in the ocean (Hayase et al., 1988, Chen and Bada, 1992, Nelson et al., 2007, Swan et al., 2009 and Jørgensen et al., 2011) and direct observations of photobleaching of CDOM in terrestrially-impacted waters (Skoog et al., 1996, Del Vecchio and Blough, 2002, Del Vecchio and Blough, 2004a, Twardowski and Donaghay, 2002 and Helms et al., 200 cool , photobleaching of open-ocean CDOM has not been widely examined (Opsahl and Benner, 1998, Ortega-Retuerta et al., 2010 and Stubbins et al., 2012), and little has been reported with respect to the changes in the spectral distribution of Torin 1 and fluorescence during photodegradation of intracellular parasites open-ocean CDOM (Hayase et al., 1988 and Chen and Bada, 1992).
2. Material and methods
2.1. Cleaning procedures
All glassware was cleaned with ~ 1 M HCl and rinsed with MilliQ UV ultrapure grade water (Millipore), designated below as “MilliQ water”. Glassware was combusted at 450 °C for ≥ 4 h. Plasticware was cleaned with ~ 1 M HCl and rinsed with MilliQ water. Stainless steel equipment was cleaned with mild detergent and copiously rinsed with MilliQ water. All containers were rinsed several times with sample prior to filling. Filter capsules (0.1 μm pore size, Whatman PolyCap) were rinsed briefly with acetonitrile, flushed with > 20 L of MilliQ water, and conditioned with approximately 1 L of sample prior to use.